NMN has been able to suppress age-associated weight gain, enhance energy metabolism and physical activity, improve insulin sensitivity, improve eye function, improve mitochondrial metabolism and prevent age-linked changes in gene expression.

NMN supplementation can reinstate NAD+ levels in the body through biosynthesis pathways, reversing aging and preventing age-associated diseases.

Taking the human and animal studies of efficacy and safety into consideration and calculating optimal NMN doses, the recommended dose adults between 30 and 60 years old take is 500 mg per day. People over age 65 can safely take 750 mg per day to maximize NMN's benefits.

Noted researcher David Sinclair of Harvard University, whose research on resveratrol, NAD+, and sirtuins is world renowned, is also conducting human trials. He is taking NMN himself; he has said his lipid profile has improved dramatically and he feels more energetic and his blood markers, at nearly 60 years old, are closer to those of a 31-year-old.

Other early adopters include Author/Personal development guru Tony Robbins

Why is NAD+ important?

NAD+ is abundant in mitochondria, the cell cytoplasm, and the nucleus. It's required for the addition of poly-ADP ribose to proteins and the deacetylating activity of sirtuin enzymes, which are critical for the reproduction of DNA sequencing regulating cell growth, energy metabolism, stress resistance, inflammation, circadian rhythm, and neural function.

NAD+ is synthesized from NMN, tryptophan, nicotinic acid, nicotinamide riboside, and nicotinamide. 

NAD+ is found in small amounts in natural foods. Such as; cow’s milk, vegetables, and meats. These precursors enter cells by different means. NMN is transported across cell membranes by the Slc12a8 transporter. It then enters cells via nicotinamide riboside transporters, and nicotinamide diffuses into cells.

The uptake of NAD+ precursors varies between tissues, but a decline in NAD+ with age is observed across multiple tissues (see image). The average NAD+ concentration in human samples is several times lower in adults than in newborns. This fall in NAD+ levels has been attributed to reduced synthesis and increased consumption, with the latter including degradation by NADase CD38 (ref. 6) and poly(ADP-ribose) polymerase (PARP).

Reduced NAD+ levels are associated with a wide range of the hallmarks of aging, from wrinkles to metabolic disorders and neurodegenerative diseases. Moreover, various lifespan-extending metabolic manipulations in mice, such as exercise, caloric restriction, and regular sleeping patterns, work in part by increasing NAD+ levels, providing further support to the idea that stimulating NAD+ metabolism could help to extend human healthspan and, potentially, lifespan.